| 产品介绍 |
人类心肌细胞通过强健且可重复的分化过程，源自人类胚胎干细胞（hESCs）。这些高度功能细胞表现出典型的心肌细胞形态、自发收缩活动以及关键心脏标志物的表达，包括cTnI和α-肌动蛋白。 它们专为体外心脏研究设计，非常适合药物筛查、心脏毒性检测、疾病建模、心脏生理学研究和组织工程。解冻后，细胞在24至72小时内附着并恢复，通常在3至5天内开始自发跳动，之后保持稳定同步的收缩。 凭借卓越的可扩展性、一致性和与人类相关的表型，这些hESC衍生心肌细胞为原发心肌细胞和动物模型提供了可靠的替代方案。 关键：心肌细胞的收缩力高度依赖于细胞密度。推荐的播种密度为2.1 ×10⁵细胞/平方厘米，以促进细胞间足够的耦合并形成同步、自发跳动的单层。较低的播种密度可能不支持可检测的拍打。 请在此查看性能数据和验证图像。 |
| 保存条件 |
Vapor phase of liquid nitrogen, or below -130°C. |
| 物种 | Human (H. sapiens) |
| 组织来源 | Heart |
| 产品形式 | Frozen |
| 预期用途 | This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. |
| 生物安全等级 | II |
| 分析证书 | For batch-specific test results, refer to the applicable certificate of analysis that can be found at www.abmgood.com. |
| 培养条件 | For optimal post-thaw recovery and culture, coat culture vessels with 1% 3DCelMatrix™ (TM076) using cold DMEM/F-12 Medium (TM510). Incubate coated vessels at 37°C for 2 hours prior to use. Immediately before plating, aspirate the coating solution and add pre-warmed Human Cardiomyocyte Maintenance Medium Kit (TM212) supplemented 10 µM ROCK Inhibitor Y-27632 (TM131) with 1% Penicillin/Streptomycin Solution (G255). Culture cells at 37°C, 5% CO₂. Perform a gentle medium change (without ROCK Inhibitor) 24 hours post-thaw to remove debris and dead cells, taking care not to disturb weakly attached cells. Replace medium every other day with fresh Human Cardiomyocyte Maintenance Medium Kit. Cells attach and recover within 24–72 hours post-thaw and begin spontaneous beating within 3–5 days, with stable, synchronous contractions maintained thereafter. |
| 表达特征 | cTnI and α-actinin |
| 应用领域 | Research Use Only. |
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